Journal: eLife

Article Title: Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

doi: 10.7554/eLife.90679

Figure Lengend Snippet: Representative immunofluorescence imaging of a half vessel confocal image stack of IALV stained for CKIT (green), CD34 (red), and DAPI (blue) counterstained as shown in Figure 2D . Image acquisition at 1 µm step interval.

Article Snippet: Antibody , anti-CKIT , Cell Signaling , Cell Signaling Technology Cat# 3074, RRID: AB_1147633 , (IF) 1:100.

Techniques:

Journal: eLife

Article Title: Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

doi: 10.7554/eLife.90679

Figure Lengend Snippet: Representative immunofluorescence imaging of a half vessel confocal stack of IALV stained for CKIT (green), CD34 (red), and VIMENTIN (cyan) as shown in Figure 2L . Image acquisition at 1 µm step interval.

Article Snippet: Antibody , anti-CKIT , Cell Signaling , Cell Signaling Technology Cat# 3074, RRID: AB_1147633 , (IF) 1:100.

Techniques:

Journal: eLife

Article Title: Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

doi: 10.7554/eLife.90679

Figure Lengend Snippet: Isolated, cannulated, and spontaneously contracting popliteal lymphatic collecting lymphatic vessel (cLV) from a CkitCreER T2 -ChR2 mouse was stimulated with light at regions of ChR2 expression, as confirmed by tdTomato imaging, but these stimulations failed to initiate a contraction. Vessels maintained spontaneous contractile activity, but contraction frequency was depressed with application of 100 nM pinacidil to ensure sufficient time in diastole for photo-stimulation. Video of the representative trace in Figure 8 .

Article Snippet: Antibody , anti-CKIT , Cell Signaling , Cell Signaling Technology Cat# 3074, RRID: AB_1147633 , (IF) 1:100.

Techniques: